OBJECTIVE: Carbapenem resistance is a major and an on-going public health problem globally. This type of antimicrobial resistance, especially when mediated by transferable carbapenemase-encoding genes, is spreading rapidly causing serious outbreaks and dramatically limiting treatment options. Limited data are regarding infection caused by Carbapenem-resistant organisms (CRO) in Dr. Kariadi Hospital.                                                                                                                                    METHOD: We conducted descriptive study by retrieving data from Microbiology Laboratory  and medical records. Subjects were patients, aged 0-17 year-old, admitted to Pediatric Intensive Care Unit (PICU) and Neonatal Intensive Care Unit (NICU) between January 2015 and December 2018. Data analysis was carried out using chi-square of Fischer-exact where appropriate and logistic regression.                                                                                                                   RESULT: In total from 2015-2018, CROs were identified at 24 (1,8%) Out of 1.268 patients who had a positive culture. The prevalence of CRO increased from (1,3%) in 2015– to (2%)  in 2018. The most frequently isolated CRO species was Klebsiella Pneumoniae (54%).  The identified risk factors for CRO infection were ICU stay >15 days (OR 3.5, 95% CI 1.004 – 11.186; p=0.046) and mechanical ventilation (OR 3.8, 95% CI 1.108 – 13.468; p=0.032). CONCLUSIONS: The increasing incidence of CRO infection in PICU and NICU of Dr. Kariadi Hospital is a serious threat for patient care. Further ICU stay > 15 days and mechanical Ventilation are important risk factors. Prudent antibiotic use,   the application of strict infection control measures, and the active surveillance for the presence of CRO infection  are of the outmost importance to be implemented.