OBJECTIVE: Infectious disease was one of the predominant cases among pediatric inpatients in developing countries. The routine evaluation of the microbiological culture is important to evaluate the treatment pattern and guidelines in every hospital. The objective of this study was to analyze the result of the microbiological culture of the urine specimens in the pediatric ward of Dr. Soetomo Hospital in 2018.                                                                                                                      METHODS: This was a cross-sectional study, analyzing the microbiology data which recorded routinely on a daily basis from all patients at the pediatric ward of Dr. Soetomo Hospital from January until December 2018. This study only presented the results from urine specimens and the bacteria were focused on Escherichia coli, Klebsiella pneumonia, and Acinetobacter baumanii. Antibiotic sensitivity tests were also performed.                                                                                                  RESULTS: There was 135 positive urine culture in 2018 comprises of 75, 51, and 9 cases of E. Coli, K. pneumoniae, and A. Baumanii, respectively. Most of K. pneumoniae and 42% of E. coli were ESBL+. Most of the patients were girls and below 5 years of age.  Among all positive cases, 32.6% were noninfectious and 17.8% were belonged to oncology group. Almost all of the isolates were resistant to ampicillin and amoxicillin-clavulanate. The resistance rates to ceftriaxone and cefotaxime were around 50%. Most isolates were sensitive to meropenem. Multi-resistant A. Baumanii was also found.                                                                                                                                  CONCLUSION: There were a high number of ESBL+ isolates from urine culture in the pediatric ward. Meropenem can be used for most cases.