Introduction: Children with Nephrotic syndrome are at increased risk of vaccine-preventable infections due to disease-related immune dysfunction and the effects of immunosuppressive therapy . Altered immune responses and concerns about vaccine safety during immunosuppression may delay or prevent timely immunization. Therefore, adequate parental knowledge regarding the disease and recommended vaccinations is essential. This study aimed to assess the level of knowledge about Nephrotic syndrome and vaccinations among parents of affected children. Methodology: A descriptive, cross-sectional quantitative study was conducted among 196 parents at Teaching Hospital Peradeniya. Data were collected using a validated selfadministered questionnaire until the required sample was achieved and entered into Microsoft Excel and analyzed using R software. A p-value