Introduction: Vitamin D (VitD) deficiency is common in children with chronic kidney disease (CKD) with a reported prevalence of 28-58%. It is a modifiable risk factor that contributes to bone mineral dysregulation and appears to be an independent predictor of CKD progression and mortality . Prevalence varies widely among nations as well as within CKD populations. As Sri Lankan data is lacking, this study was conducted to understand the local disease burden. Objective: To determine prevalence of VitD deficiency and insufficiency in children with CKD and to evaluate association between VitD levels and glomerular filtration rate (GFR), gender, age, body mass index, parathyroid hormone levels (PTH) and stages of CKD. Method: A descriptive cross-sectional study was conducted among children with CKD attending Paediatric Nephrology clinic at National Hospital, Galle. Results: A total of 233 children with a mean age of 9.1±4.7 years and mean eGFR of 82.1±32.8ml/min/1.73m2 were included. The mean VitD level was 99.6±37nmol/L. Overall, 180(77%) had sufficient levels, while 38(16%) were insufficient and 14(6%) were deficient. One had toxic levels. Fourteen children (6%) have received VitD intramuscular injections in the past 6 months. Males (M104.6,SD=38) had significantly higher levels than females (M=87 .35,SD=31.65); t(231)=3.55,p