Introduction: Neonatal resuscitation is a critical component of essential newborn care. Sri Lanka National Neonatal Life Support (NLS) guidelines recommend that all healthcare staff attending deliveries are adequately trained, confident, and supported. Objective: To assess knowledge, training, confidence, and clinical practice related to neonatalresuscitation and neonatal life support among healthcare staff at DGH Ampara. Methodology: A descriptive clinical audit was conducted using a structured questionnaire among 60 doctors, nurses, and midwives involved in neonatal care in NICU, postnatal wards, labour rooms, and operating theatres at DGH Ampara. Results: Of the participants, 12% were doctors, 82% nurses, and 5% midwives. Staff with >10 years ofexperience accounted for 44%, while 28% had 85% (excellent/fully competent), 16% scored 75–84% (good/largely competent), 57% scored 50–74% (fair/partially competent), and 13%scored